A Root-Expressed l-Phenylalanine:4-Hydroxyphenylpyruvate Aminotransferase Is Required for Tropane Alkaloid Biosynthesis in Atropa belladonna
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چکیده
منابع مشابه
A root-expressed L-phenylalanine:4-hydroxyphenylpyruvate aminotransferase is required for tropane alkaloid biosynthesis in Atropa belladonna.
The tropane alkaloids, hyoscyamine and scopolamine, are medicinal compounds that are the active components of several therapeutics. Hyoscyamine and scopolamine are synthesized in the roots of specific genera of the Solanaceae in a multistep pathway that is only partially elucidated. To facilitate greater understanding of tropane alkaloid biosynthesis, a de novo transcriptome assembly was develo...
متن کاملEnhancement of Tropane Alkaloid Production among Several Clones and Explants Types of Hairy Root of Atropa belladonna L.
Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...
متن کاملenhancement of tropane alkaloid production among several clones and explants types of hairy root of atropa belladonna l.
agrobacterium rhizogenes (pri), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. in our study four bacterial strains ar15834, a4, 9435 and c318 and three explants types leaf, stems and roots, were examined. hairy roots were induced from roots, stems and leaf explants. the highest transformation efficiency of 77% was achieved by usi...
متن کاملOverexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures.
The medicinally applied tropane alkaloids hyoscyamine and scopolamine are produced in Atropa belladonna L. and in a small number of other Solanaceae. Calystegines are nortropane alkaloids that derive from a branching point in the tropane alkaloid biosynthetic pathway. In A. belladonna root cultures, calystegine molar concentration is 2-fold higher than that of hyoscyamine and scopolamine. In th...
متن کاملEffect of Gamma Radiation and Gibberellic Acid on Germination and Alkaloid Production in Atropa belladonna l
This investigation was carried out to improve the germination percentage and alkaloid 1 production in M seeds (irradiated seeds with 50, 80, 110 and 150 Gy) of Atropa belladonna L. by using and different concentrations of gibberellic acid (20,40,60,80,100 and 120 ppm). There was an increase in seed germination percentage by increasing gamma-ray doses until 150 Gy which gave 3 decrease in seed g...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 2014
ISSN: 1532-298X,1040-4651
DOI: 10.1105/tpc.114.130534